Salindri Prawitasari

Name   : Salindri Prawitasari

Batch    : 2019

E-mail   : prawitasarisalindri@gmail.com

Interest : Medical biotechnology, stem cell research, exosome, regenerative therapy, research and development.

Research Experience

My research focuses on the optimization of exosome derived from human Wharton’s Jelly Mesenchymal Stem Cells (hWJ-MSCs) through hWJ-MSCs preconditioning. hWJ-MSCs preconditioned with hypoxic condition and addition of L=Ascorbic Acid. Moreover, I characterized the exosome morphology by Transmission Electron Microscopy (TEM), determined the exosome concentration and particle size using Nanoparticle Tracking Analysis (NTA), and observed exosome function using immunocytochemistry. This research was aimed to induce hWJ-MSCs differentiation to chondrocytes.

Skills

Stem cell culture (from primary culture) and characterization, cell thawing, cell counting, cell passaging, cell cryopreservation, cell staining. MTT assay, BCA assay, UV-Vis Spectrophotometry, immunocytochemistry. Exosome isolation, characterization, and internalization. Statistical analysis using SPSS and GraphPad Prism software.

Testimonial

Biotechnology master’s program is a very supportive place to improve academic and non-academic skills. The lecturers are very competent in their majors so they can support students’ interests and talents. The campus environment encourages students to be disciplined and work in a fast-paced rhythm. In addition, students are offered various kind of internship, exchange, and scholarship program. Thank you Biotechnology ITB for a very impressive learning experience!