Siti Sarah

Name   : Siti Sarah
Batch : 202o
E-mail   : sitisarahilyas@gmail.com
Interest : Microbial Biotechnology, Industrial Biotechnology, Bioprocess Engineering, Fermentation, Metabolite Production

Research Experience
My research focused on the utilization of pretreated lignocellulosic biomass, Oil Palm Empty Fruit Bunch (OPEFB), as a medium for lipid production by the oleaginous yeast Rhodotorula toruloides. In this research, I applied solid-state fermentation using the white-rot fungus Marasmiellus sp. for the pretreatment of OPEFB. Post-pretreatment, lipid production by R. toruloides using the pretreated OPEFB medium was conducted under various feeding conditions to optimize lipid yield. To analyze the lipid yield produced, I employed the Folch method for lipid extraction, ensuring accurate quantification of the produced lipid. This research demonstrated the feasibility of utilizing pretreated OPEFB as an alternative substrate for sustainable lipid production, contributing to the development of bioproducts derived from agro-industrial waste. 

Skills
Microbial Cultivation, Bioprocess Optimization, Lipid Extraction, Enzyme Activity Assay, UV-Vis spectrophotometry

 Testimonial
Studying in the Biotechnology program at the School of Life Sciences and Technology, Bandung Institute of Technology (ITB) has provided me with a meaningful experience. I have gained many skills and hands-on research experience in practical applications. The supportive environment, along with accessible facilities and knowledgeable lecturers, has promoted a conducive learning atmosphere.