Rizal Fanany

Name   : Rizal Fanany
Batch : 2022
E-mail   : fananyrizal11@gmail.com
Interest : Recombinant protein expression in E. coli, Bioprocess engineering, and Bioinformatic

Research Experience
During my Master’s studies, my research focused on recombinant protein expression in Escherichia coli BL21. My thesis, titled Construction and Optimisation of Bst DNA Polymerase Enzyme Production in Escherichia coli BL21 (DE3), aimed to express and optimize the production of the Bst DNA polymerase enzyme in E. coli BL21. Through this research, I gained fundamental skills in expression vector cloning, transformation, production, and purification of recombinant proteins in Escherichia coli. I also employed bioprocess engineering approaches to optimize protein production.

In addition, I completed a bioinformatics mini-project titled Anti-viral Drug Discovery Against Human Monkeypox Virus (MPXV) by Local Natural Compounds: An Ethno-Bioinformatic Drug Design Approach, where I developed skills in 3D protein modeling, molecular docking, and drug-like compound analysis. 

Skills
• DNA, RNA, and Bacterial Plasmid Extraction
• Polymerase Chain Reaction (PCR) & qPCR
• Loop-Mediated Isothermal Amplification (LAMP)
• Cloning of expression vectors
• Plasmid Transformation in Escherichia coli host
• Protein expression in Escherichia coli host
• Protein Purification from Escherichia coli host
• Agarose Gel Electrophoresis & Sodium Dodecyl Sulfate Polyacrylamide Gel (SDS-PAGE) Electrophoresis
• Primer Design in PCR and LAMP
• Multiple Alignment and Phylogenetic Analysis
• 3D Molecular Protein Structure
• Molecular Docking & Analysis of Drug-Like Compounds
• Statistical Analysis using R and SPSS

Testimonial
The Biotechnology study program at SITH ITB has greatly supported me in acquiring a wide range of skills in the biotechnology field, particularly in molecular biology and bioprocess engineering. The learning process has been challenging and enjoyable with comprehensive laboratory facilities, highly competent lecturers, and a supportive academic environment.
I want to express my gratitude to my lecturers, thesis supervisors, faculty staff, and fellow students who have made this academic experience both valuable and memorable.