Agika Manjaswari

Name   : Agika Manjaswari
Batch : 2022
E-mail   : agikamanjaswari12@gmail.com
Interest :  Protein engineering, enzyme production, microbial biotechnology, fermentation technology, recombinant DNA technology, and industrial bioprocess optimization.

Research Experience
My recent research has focused on the development of a streamlined production and purification strategy for recombinant Taq DNA polymerase in E. coli BL21(DE3). Through optimization of temperature, induction conditions, and purification methods, I successfully reduced process time while maintaining enzyme activity. This research highlights my ability to manage complex biotechnological projects, from molecular cloning using Gibson Assembly to the development of efficient purification techniques, such as solvent precipitation. The research resulted in a significantly higher yield of functional Taq DNA polymerase, making the process more time-efficient and effective for potential industrial applications.

Skills
Protein production and purification, molecular cloning, recombinant DNA technology, microbial fermentation, enzyme activity assays, process optimization for large-scale production, SDS-PAGE, UV-Vis spectrophotometry, PCR, solvent precipitation, chromatography, and basic bioinformatics tools.

Testimonial
Studying in the Master of Biotechnology program at SITH ITB has provided me with a deep understanding of biotechnology and bioprocesses, particularly in recombinant protein production and purification. Throughout the coursework, I gained not only strong theoretical knowledge but also practical experience in developing and optimizing biotechnological processes. Additionally, I was given the opportunity to teach and train students for the national and international Biology Olympiads. This experience enhanced my leadership and communication skills, while also strengthening my ability to transfer scientific knowledge to the younger generation.